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Type: 
Journal
Description: 
This paper describes the optimisation and the analytical performances of a label-free impedimetric immunosensor for the detection of tumour marker CA125 based on gold nanoparticles modified screen-printed graphite electrode. Experimental conditions of each step for the developed immunosensor were studied and optimised. The immunosensor response varied linearly (r2 = 0.996) with antigen concentration between 0 and 100 U/mL. The estimated detection limit was 6.7 U/mL. The electrochemical immunosensor allowed unambiguous identification of CA125, while no significant non-specific signal was detected in the case of all negative controls. The analytical usefulness of the impedimetric immunosensor was finally demonstrated analysing serum samples.
Publisher: 
Elsevier
Publication date: 
31 Mar 2013
Authors: 

A Ravalli, G Pilon Dos Santos, M Ferroni, G Faglia, Hideko Yamanaka, G Marrazza

Biblio References: 
Volume: 179 Pages: 194-200
Origin: 
Sensors and Actuators B: Chemical