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CdnL is an essential RNA polymerase (RNAP)-binding activator of rRNA transcription in mycobacteria and myxobacteria but reportedly not in Bacillus. Whether its function and mode of action are conserved in other bacteria thus remains unclear. Because virtually all alphaproteobacteria have a CdnL homolog and none of these have been characterized, we studied the homolog (CdnL Cc) of the model alphaproteobacterium Caulobacter crescentus. We show that CdnL Cc is not essential for viability but that its absence or depletion causes slow growth and cell filamentation. CdnL Cc is degraded in vivo in a manner dependent on its C-terminus, yet excess CdnL Cc resulting from its stabilization did not adversely affect growth. We find that CdnL Cc interacts with itself and with the RNAP β subunit, and localizes to at least one rRNA promoter in vivo, whose activity diminishes upon depletion of CdnL Cc. Interestingly …
Nature Publishing Group
Publication date: 
24 Feb 2017

Aránzazu Gallego-García, Antonio A Iniesta, Diego González, Justine Collier, Subramanian Padmanabhan, Montserrat Elías-Arnanz

Biblio References: 
Volume: 7 Issue: 1 Pages: 1-16
Scientific reports